Table 2. Microbiological data for possums with clinical M. ulcerans infection.

Case:	IS2404 PCR results:	Culture results:	Typing results:
1	PCR +ve: nose lesion	Culture +ve: nose lesion	NA
2	PCR +ve: nose lesion	Culture +ve: nose lesion	NA
3	PCR +ve: site not specified	Culture +ve: site not specified	NA
4	PCR +ve: site not specified	Not cultured	NA
5	PCR +ve: lesion swab and stomach wall. PCR −ve: liver	Culture +ve: facial ulcer tissue. Culture −ve: liver and stomach wall	RFLP ‘V1’
6	PCR +ve: site not specified	Culture +ve: site not specified	RFLP ‘V1’nVNTR ‘Victorian’
7	PCR +ve: site not specified	Culture +ve: site not specified	RFLP ‘V1’ VNTR ‘Victorian’
8	PCR +ve: tail base and mid-tail lesions	Culture +ve: both tail lesions	RFLP ‘V1’
9	PCR +ve: site not specified	Culture contaminated	NA
10	PCR +ve: site not specified	Culture +ve: left hind foot lesion	VNTR ‘Victorian’ WGS
11	PCR +ve: site not specified	Culture +ve: site not specified	VNTR ‘Victorian’
12	PCR +ve: tail and toe lesions, buccal swab. PCR −ve: blood sample	Culture +ve: toe and tail lesions	VNTR ‘Victorian’
13	PCR +ve: tail lesions. PCR −ve blood, faeces, cloacal and nasal swab	Culture not performed	NA
14	PCR +ve: tail lesion, gut contents, faeces. PCR −ve: buccal and nasal swabs, blood, bile, urine, salivary gland, lung, liver, spleen, kidney	Culture +ve: tail lesion. Culture −ve: gut contents	VNTR ‘Victorian’
15	PCR +ve: tail lesion, faeces, salivary gland, gut contents. PCR −ve: buccal and nasal swabs, blood, bile, urine, lung, liver, spleen, kidney	Culture +ve: tail lesion. Culture −ve: salivary gland, gut contents	VNTR ‘Victorian’
16	PCR +ve: multiple limb lesions, facial lesions, tail lesion, lung, liver, spleen, kidney, mandibular lymph node, heart, mesenteric lymph node, salivary gland, buccal swab, gut contents	Culture +ve: liver, nose lesion, mandibular lymph node, spleen. Culture −ve: hind leg muscle, colon, right hock,stomach tissue, gut contents	VNTR ‘Victorian’ WGS
17	PCR +ve: from swab of multiple combined sites	Culture +ve: swab of multiple combined sites	VNTR ‘Victorian’
18	PCR +ve: ear tissue	Culture −ve: ear tissue	NA
19	PCR +ve: faeces, toe lesion. PCR −ve: blood, buccal and nasal swab	Culture not performed	NA
20	PCR +ve: toe lesion, faeces. PCR −ve: blood, buccal and pouch swabs	Culture +ve: toe lesion	VNTR ‘Victorian’
21	PCR +ve: toe lesion, faeces, buccal swab. PCR −ve: blood	Culture −ve: toe lesion	NA
22	PCR +ve: tail lesion, faeces, buccal swab. PCR −ve: blood	Culture +ve: tail lesion	VNTR ‘Victorian’
23	PCR +ve: pouch, buccal swab, nasal swab, tail and, ear lesion swabs, faeces. PCR −ve: blood	Culture not performed	NA
24	PCR +ve: faeces, tail,eye and nose lesions, buccal, and pouch swabs, spleen, lung, kidney, liver, gut contents	Culture +ve: tail lesion. Culture −ve: gut contents	VNTR ‘Victorian’
25	PCR +ve: eye lesion, nasal lesion, lung, liver, spleen, kidney, buccal swab, gut contents	Culture +ve: tail lesion. Culture −ve: gut contents	VNTR ‘Victorian’
26	PCR +ve: tail lesion, buccal. PCR −ve: clocal swab	Culture +ve: caudal abdominal cavity	VNTR ‘Victorian’
27	PCR +ve: tail and hind leg lesions, liver, lung, gut contents	Culture +ve: tail and hind leg lesions, liver, lung and SI contents. Culture −ve: stomach, caecum and LI contents	VNTR ‘Victorian’

IS2404 PCR = polymerase chain reaction targeted against insertion sequence 2404, +ve = positive, −ve = negative, NA = not available, RFLP = restriction fragment length polymorphism, VNTR = variable numbers tandem repeats, WGS =  whole genome sequencing, SI = small intestinal, LI = large intestinal, real-time PCR cycle threshold (C_T): (+/−) = C_T >35, (+) = C_T >30–35, (++) = C_T >25–30, (+++) = C_T >20–25, (++++) C_T≤20.