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. 2014 Jan 30;9(1):e85123. doi: 10.1371/journal.pone.0085123

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(a) Expression of miR-21 in mammary glands from virgin and 6 day pregnant Stat5^fl/fl and Stat5^{fl/fl;MMTV-Cre} mice. (b) Expression of miR-21 in Stat5^fl/fl mice in mammary glands from virgin and 6 day pregnant mice. (c) Expression of beta-casein in Stat5^{fl/fl;MMTV-Cre} mice in mammary glands from virgin and 6 day pregnant mice. (d) Expression of beta-casein in Stat5^fl/fl mice in mammary glands from virgin and 6 day pregnant mice. The miRNA levels were normalized to U6 levels, mRNA levels were normalized to beta-actin mRNA levels. In each analysis the virgin mammary gland was used as the control sample (fold change = 1). Values are means ± S.D. for three independent experiments. (e) Detection of mir-21 expression in the mammary gland of 6 day pregnant mice. In situ hybridization analysis using DIG-conjugated, LNA-modified DNA probes complementary to U6 (right), miR-21 (middle) and scrambled (left). Detection was performed on formalin-fixed, paraffin-embedded section from 6 day pregnant mammary glands.

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