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. 2014 Jan 30;8(1):e2676. doi: 10.1371/journal.pntd.0002676

Figure 4. Effect of metal ions on the phosphatase activity of rTcCaNA2 and TcCaNA2/TcCaNB.

Figure 4

A) Catalytic activity was evaluated in the presence of the divalent cations at 1 mM, using 80 mM p-NPP as substrate and 1 µg rTcCaNA2. All data are presented as means ± standard error of the mean (SEM) of triplicates. Statistical significance was determined by Tukey's Multiple Comparison Test (p<0.05). **p = 0.0015 and *p<0.05 in relation to the activity without metal ions. The results are representative of two independent experiments performed in triplicate. B) Catalytic activity of TcCaNA2 was evaluated as in A in the presence of rTcCaNB and/or EGTA. Statistical significance was determined by Tukey's Multiple Comparison Test (p<0.05). *p<0.05 in relation to the activity with only rTcCaNA2 and **p<0.05 in relation to activity of rTcCaNA2/rTcCaNB heterodimer. The results are representative of two independent experiments performed in triplicate.