Figure 4. Effect of glycyrrhizin on the expression of HBD-1 mRNA and HBD-1 production by patient unburned skin tissues transwell-cultured with the same patient CD31⁺ IMC.

A. mRNA expression. Patient #11 unburned skin tissues (0.2×0.2 cm, lower chamber) were transwell-cultured with their CD31⁺ IMC (1×10⁵ cells/ml, upper chamber) for 48 hours in the presence or absence of 1 to 100 µg/ml of glycyrrhizin. Skin tissues harvested were analyzed for HBD-1 mRNA by RT-PCR. Data are representative of 4 individual experiments using unburned skin tissues and CD31⁺ IMC obtained from patients #12∼#15. B. HBD-1 production. Transwell-cultures between unburned skin tissues from patients #11∼#15 and their CD31⁺ IMC were respectively performed in the presence of 100 µg/ml of glycyrrhizin. Culture fluids harvested 48 hours after cultivation were assayed for HBD-1 by ELISA.