Figure 1. Stage specific inhibition of Bmp in K. marmoratus.

Embryos were exposed to 100 µM dorsomorphin at the 32-cell (B), late blastula (C) and 80% epiboly (D) stages of development. Photographs of the embryos were taken 3 days post-fertilization. A1–3: Control (n = 20/20). B1–3: splitbody (phenotype variation details in Figure 3), this phenotype is characterised by absence of a distinct tail region (B1 arrowhead), morphologically undifferentiated head region (B2 arrowhead) and split body axis (B2 arrow), and cell islands in the posterior region (B3 arrowhead). C1–3: Curled tail (n = 8/10), this phenotype resembles snailhouse seen in zebrafish and is characterised by its curled tail (C3 arrowhead). D1–3: Bent tail (n = 12/12, this phenotype primarily displayed a bent tail (D3 arrowhead). Overview images are lateral views and head/tail images are dorsal views of the embryos. Scale bars: 250 µm.