Table 2. Real-time RT-PCR, H&E and IHC result comparison for goats euthanized on 6, 8 and 11 days post-infection.
| Goat DPI6 | Goat DPI8 | Goat DPI11 | |||||||
| Tissues | RNA copy/g | H&E | IHC | RNA copy/g | H&E | IHC | RNA copy/g | H&E | IHC |
| (log10) | (log10) | (log10) | |||||||
| Parotid LN | 3.8 | Y | +++ | 5.1 | Y | ++ | 3.0 | Y | + wk |
| Retropharyngeal LN | 4.3 | Y | ++ | 5.0 | Y | + wk | 3.9 | Y | + wk |
| Bronchial LN | 3.4 | Y | ++++ | 4.7 | Y | ++ | 3.5 | Y | + wk |
| Prescapular LN | 4.6 | Y | ++ | 4.5 | Y | ++ | 3.3 | Y | + wk |
| Mesenteric LN | 5.0 | Y | ++ | 4.1 | Y | +++ | 4.5 | Y | + wk |
| Mandibular LN | 4.6 | Y | ++ | 4.4 | Y | ++ | 4.7 | Y | + wk |
| Lung – cranial | 4.5 | Y | + | 5.2 | Y | +++ | − | Y | − |
| Liver | − | N | − | 2.1 | Y | + wk | 3.1 | N | − |
| Spleen | − | Y | +++ | 4.0 | Y | ++ | 2.8 | N | + wk |
| Palentine Tonsil | 5.5 | Y | +++ | 5.4 | Y | ++++ | 3.8 | NS | NS |
| Skin/Lip | 3.4 | N | + wk | 2.9 | Y | +++ | − | N | − |
| Tongue | − | N | − | − | Y | ++++ | − | N | − |
| Trachea | 3.6 | Y | + | 5.2 | Y | +++ | − | Y | − |
| Abomasum | − | N | + wk | 4.3 | Y | ++ | − | N | − |
| Rumen | 3.6 | N | − | − | Y | + wk | 4.8 | Y | + wk |
| Omasum | − | N | − | − | Y | − | 5.3 | N | − |
| Duodenum | 6.0 | Y | + wk | 3.4 | Y | +++ | 3.0 | Y | + wk |
| Ileum | 6.3 | Y | +++ | 3.9 | Y | ++++ | 3.0 | Y | + wk |
| Caecum | 5.5 | Y | ++ | 7.4 | Y | ++++ | 2.8 | Y | − |
| Colon | 5.5 | Y | ++ | 5.2 | Y | ++++ | − | Y | − |
| Conjunctiva | 4.4 | N | − | 5.7 | Y | ++ | − | Y | + wk |
| 3rd eyelid | 4.4 | Y | + | 3.3 | Y | ++ | − | Y | + wk |
| Pharyngeal mucosa | 3.6 | N | − | 4.3 | Y | + | − | N | − |
| Oral mucosa | − | Y | + wk | 7.8 | Y | +++ | − | N | − |
| Nasal mucosa | 6.2 | Y | + wk | 3.8 | Y | +++ | 4.1 | Y | + wk |
Y = Lesions present consistent with PPR infection.
N = No significant histopathological findings.
NS− No sample.
IHC = Immunohistochemistry score: +wk = weak immunostaining (<20 cells);+ = mild immunostaining (<25% of the section);++ = moderate immunostaining (25% to 50% of the section);+++ = abundant immunostaining (51% to 75% of the section);++++ = extensive immunostaining (>75% of the section).