Figure 2. Hook1 and Rab22a colocalize with CIE cargo on recycling tubular endosomes. HeLa cells expressing endogenous levels of Hook1 and overexpressing the constitutively active Rab22a mutant (GFP-Rab22a Q64L) were incubated with anti-CD147 antibody for 30 min at 37°C to allow internalization of bound antibodies. Hook1, Rab22a and CD147 meet on puncta and tubular endosomal structures after internalization.