Figure 1. Inhibition of RNA editing by dual RNAi knockdowns of critical subunits in the RNA editing core (MP18 and MP24) and guide RNA binding (GRBC1 and GRBC2) complexes, and RNAi-based knock-in of catalytically inactive RET2 TUTase. Change in relative abundance for mitochondrial mRNAs and rRNAs was determined by real-time RT-PCR analysis; thick line at “1” reflects no change in relative abundance upon RNAi induction; bars above or below represent an increase or decrease, respectively. RNA levels were normalized to β-tubulin mRNA. Error bars represent the standard deviation from at least three replicates. P, pre-edited mRNA; E, edited mRNA; n/d, not determined.