Figure 3. Loss of the 80S mRNA-associated ribosomal population upon inactivation of RNA editing. (A) Native gel analysis of ribosomal complexes. Total cell lysates obtained from parental and RET2 knock-in cell lines were fractioned on glycerol gradients and each fraction was further separated on 3–12% Bis-Tris NuPAGE native gel and transferred onto nylon membrane. NativeMARK Unstained Protein Standards (Invitrogen) were separated alongside. Hybridizations were performed with the same probes as in Figure 2 to detect fully-edited RPS12 mRNA, 9S, and 12S rRNAs. (B) Sedimentation of rRNAs. RNA was extracted from indicated fractions, separated on 5% polyacrylamide/8M urea gel and simultaneously probed for 9S and 12S rRNAs.