Figure 4. Reduction of the 45S SSU-related complex (SSU*) and the 80S complex upon inactivation of RNA editing. (A) Ribosomal complexes in RET2 RNAi uninduced cells (tet[-]), as well as in cells upon induction of the RNAi at specified time points. Total cell lysates were fractionated in sucrose gradients; 9S and 12S rRNAs in gradient fractions were analyzed by slot-blot hybridization. (B) Ribosomal complexes in RET2-CODA WT and RET2-CODA D97A knock-in cells.