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. 2014 Jan 30;9(1):e87717. doi: 10.1371/journal.pone.0087717

Figure 1. iNKT cells reside in the CNS in the absence of inflammation.

Figure 1

(A) Mononuclear cells were isolated from the brain and spinal cord (left), spleen (middle), and liver (right) of unmanipulated adult C57Bl/6 (top) or Jα18-/- C57Bl/6 mice (bottom) by percoll gradient. A representative CD1d:α-GalCer tetramer staining on mononuclear cells is presented. (B) Frequency of CD1d:α-GalCer tetramer positive cells among αβ T cells in the liver (n = 6), spleen (n = 7), peripheral blood (n = 5), CNS (n = 7, 3–16 pooled tissues/observation). (C) Absolute numbers of CD1d:α-GalCer tetramer positive αβ T cells in the liver (n = 5) and CNS (n = 7, 3–16 pooled tissues/observation) among percoll purified mononuclear cells. Data represent the mean ± SEM of n individual mice per group.