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. 2014 Jan 30;9(1):e87787. doi: 10.1371/journal.pone.0087787

Figure 6. IL-35 expression in ApoE−/− mice with atherosclerotic plaque.

Figure 6

A. Serum was obtained from C57BL/6 and ApoE−/− mice at 8 weeks of age fed with normal diet, without plaque (8 weeks) and at 16 weeks of age, after 8 weeks on a high-fat diet, C57BL/6 without plaque and ApoE−/− mice with established plaque (16 weeks). The concentrations of IL-35 (pg/mL) in serum were detected by ELISA. B. Total RNA was isolated from thoracic and abdominal aorta of C57BL/6 and ApoE−/− mice at 8 weeks of age fed with normal diet, without plaque (8 weeks) and at 16 weeks of age, for 8 weeks after high-fat diet, C57BL/6 without plaque and ApoE−/− mice with established plaque (16 weeks). The expression of IL-35-related subunits was analyzed by real-time PCR. (n = 3 per group), *p<0.05,***p<0.001.