Figure 4. Endogenous N. benthamiana SnRK1 activity co-purifies with expressed ADK.

(A) HA₂His₆-tagged Arabidopsis ADK and HA₂His₆-GFP (control) were expressed in N. benthamiana and enriched using nickel-NTA resin. Proteins were fractionated by SDS-PAGE, and Coomassie stained samples are shown. (B) GST-SAMS and γ³²P-ATP were added to ADK or GFP preparations (3 µg protein). Samples were separated by SDS-PAGE and exposed to a phosphor-imager for 2 days. The Coomassie stained panel is a loading control for GST-SAMS and GST-SAMA, while the immunoblot (bottom panel, probed with anti-HA) is a loading control for ADK and GFP.