Table 1. ADK interacts with SnRK1 and SnRK1-KD in yeast cells.

Bait	Prey	Interaction
SnRK1	ADK	+
ADK	SnRK1	+
SnRK1-KD	ADK	+
ADK	SnRK1-KD	+
ADK	CAT	-
ADK	p53	-
SnRK1	CAT	-
SnRK1	p53	-

The indicated bait proteins were expressed as GAL4 DNA binding domain fusions, and the prey proteins as GAL4 activation domain fusions, in yeast Y190 cells. SnRK1 indicates the full-length protein and SnRK1-KD denotes the kinase domain. Chloramphenicol acetyl transferase (CAT) and p53 were negative controls. Interaction was indicated by cell growth on medium lacking histidine and containing 50 mM aminotriazole. As an additional indicator of interaction, colonies were monitored for LacZ activity (blue color) using a filter-lift assay.