. 2014 Jan 30;9(1):e87374. doi: 10.1371/journal.pone.0087374

Table 3. Changes of mRNA abundance (Mean ± SEM ΔΔCt¹) of immune factors in bMEC stimulated with six heat-inactivated S. aureus strains for 1, 6, or 24 h.

		SLAT(+)³			SLAT(−)³			Analysis of variance (P-value)
Gene²	Time (h)	692	1130	1989	1586	1904	2071	Strain(Type)	Cow	Strain^*Cow
IL-1β	1	0.5±0.3 ^ac	1.6±0.2 ^b ^*	1.3±0.3 ^bc ^*	1.3±0.5 ^bc ^*	1.5±0.4 ^b ^*	0.5±0.3 ^a	0.01	<0.01	0.03
	6	8.3±0.2 ^a ^*	8.7±0.2 ^a ^*	8.3±0.3 ^a ^*	3.5±0.1 ^b ^*	5.7±0.5 ^c ^*	5.3±0.2 ^c ^*	<0.01	<0.01	0.01
	24	5.1±0.3 ^ab ^*	5.7±0.2 ^b ^*	4.8±0.1 ^a ^*	0.4±0.4 ^c	1.8±0.3 ^d ^*	1.6±0.3 ^d ^*	<0.01	<0.01	0.03
TNF-α	1	0.7±0.2 ^a	0.8±0.1 ^a	0.3±0.3 ^a	0.2±0.6 ^ab	05±0.5 ^a	−0.6±0.3 ^b	0.07	<0.01	0.02
	6	5.4±0.4 ^a ^*	5.1±0.2 ^a ^*	5.1±0.4 ^a ^*	1.3±0.6 ^b ^*	3.4±0.3 ^c ^*	2.9±0.4 ^c ^*	<0.01	<0.01	0.75
	24	4.1±0.2 ^a ^*	4.3±0.2 ^a ^*	4.1±0.1 ^a ^*	1.1±0.2 ^b ^*	2.0±0.4 ^c ^*	1.6±0.1 ^bc ^*	0.11	0.06	0.23
IL-8	1	2.0±0.5 ^a ^*	2.1±0.4 ^a ^*	1.5±0.2 ^ab ^*	0.6±0.4 ^b	0.8±0.3 ^b	0.9±0.5 ^b	0.73	<0.01	0.07
	6	7.8±0.2 ^a ^*	7.1±0.2 ^b ^*	7.0±0.4 ^b ^*	4.7±0.1 ^c ^*	6.1±0.3 ^d ^*	5.4±0.3 ^e ^*	<0.01	<0.01	0.01
	24	7.1±0.3 ^a ^*	7.0±0.2 ^a ^*	7.1±0.1 ^a ^*	3.5±0.4 ^b ^*	4.8±0.2 ^c ^*	4.1±0.6 ^c ^*	<0.01	<0.01	0.01
RANTES	1	−0.2±0.2 ^ab	−0.6±0.3 ^ab	0.1±0.5 ^a	−0.6±0.4 ^ab	−0.5±0.4 ^ab	−0.8±0.5 ^b	0.56	<0.01	0.72
	6	3.9±0.2 ^a ^*	4.3±0.2 ^a ^*	2.9±0.3 ^b ^*	0.0±0.2 ^c	1.8±0.4 ^d ^*	1.0±0.3 ^e ^*	<0.01	<0.01	0.69
	24	3.2±0.3 ^a ^*	4.0±0.3 ^b ^*	3.0±0.3 ^a ^*	−0.1±0.5 ^c	1.1±0.2 ^d ^*	0.6±0.4 ^d	<0.01	<0.01	0.06
SAA	1	−1.0±0.5 ^a	−0.6±0.4 ^ab	0.8±1.0 ^c	−0.9±0.9 ^a	0.7±0.8 ^bc	−0.9±0.6 ^a	0.01	<0.01	0.50
	6	5.6±0.6 ^a ^*	4.9±0.5 ^ab ^*	4.7±0.4 ^b ^*	0.8±0.4 ^c	3.6±0.3 ^d ^*	1.6±0.3 ^e ^*	<0.01	<0.01	<0.01
	24	5.3±0.4 ^a ^*	5.3±0.6 ^a ^*	6.3±0.5 ^b ^*	1.9±0.5 ^c ^*	3.3±0.4 ^d ^*	3.0±0.6 ^d ^*	<0.01	<0.01	0.47
Lf	1	0.7±0.2 ^a	0.1±0.3 ^bc	−0.1±0.2 ^d	0.4±0.4 ^ab	−0.7±0.4 ^d	−0.6±0.3 ^cd	0.01	<0.01	0.53
	6	2.6±0.4 ^a ^*	2.1±0.1 ^ab ^*	1.9±0.4 ^b ^*	0.0±0.5 ^c	1.6±0.2 ^b ^*	0.6±0.2 ^d	<0.01	<0.01	0.02
	24	3.9±0.5 ^a ^*	3.6±0.5 ^a ^*	4.1±0.5 ^a ^*	1.9±0.8 ^b ^*	2.4±0.4 ^b ^*	1.6±0.7 ^b ^*	0.36	<0.01	0.03
GM-CSF	1	0.6±0.2 ^a	0.0±0.4 ^ab	−0.5±0.5 ^b	0.3±0.1 ^a	0.4±0.2 ^a	0.5±0.3 ^a	0.07	0.01	0.16
	6	3.1±0.1 ^a ^*	3.9±0.2 ^b ^*	3.0±0.4 ^a ^*	0.0±0.3 ^c	1.6±0.2 ^d ^*	1.9±0.3 ^d ^*	<0.01	0.71	0.04
	24	0.4±0.6 ^ab	1.7±0.4 ^c ^*	0.5±0.4 ^a	−1.4±0.3 ^d	−0.7±0.4 ^bd	−1.0±0.6 ^d	0.06	<0.01	0.39
COX-2	1	0.5±0.3	0.3±0.2	−0.1±0.4	0.6±0.3	−0.3±0.5	0.0±0.1	0.27	0.08	0.03
	6	1.8±0.2 ^a ^*	1.8±0.4 ^ab ^*	1.5±0.3 ^abd ^*	0.2±0.1 ^c	1.1±0.1 ^bd ^*	1.0±0.4 ^d	0.06	<0.01	0.01
	24	0.1±0.5 ^ab	0.8±0.2 ^b	0.8±0.2 ^b	−0.6±0.3 ^a	−0.3±0.2 ^a	−0.4±0.4 ^a	0.58	0.59	<0.01
TLR2	1	0.0±0.3 ^a	0.6±0.3 ^a	0.3±0.9 ^a	0.6±0.3 ^a	−0.3±0.3 ^a	−0.2±0.5 ^a	0.57	0.05	0.95
	6	2.7±0.3 ^a ^*	1.9±0.2 ^b ^*	2.0±0.3 ^b ^*	0.4±0.4 ^c	1.7±0.2 ^bd ^*	1.1±0.3 ^d ^*	<0.01	<0.01	<0.01
	24	2.1±0.4 ^ab ^*	2.2±0.6 ^a ^*	2.6±0.5 ^a ^*	0.9±0.4 ^c	1.5±0.3 ^bc ^*	1.1±0.6 ^c ^*	0.23	<0.01	0.51

^a–e

Means within a row without common superscript letters differ (P<0.05).

Means differ significantly from negative control.

ΔΔCt values are normalized to negative controls and corrected for the two reference genes, GAPDH and ubiquitin.

IL-1β = interleukin-1β; TNF-α = tumor necrosis factor-α; IL-8 = interleukin-8; RANTES = regulated on activation, normal T cell expressed and secreted; SAA = serum amyloid A; Lf = lactoferrin; GM-CSF = granulocyte-macrophage colony-stimulating factor; COX-2 = cyclooxygenase-2; TLR2 = toll-like receptor 2.

SLAT(−) = S. aureus latex agglutination test negative; SLAT(+) = S. aureus latex agglutination test positive.

Table 3. Changes of mRNA abundance (Mean ± SEM ΔΔCt1) of immune factors in bMEC stimulated with six heat-inactivated S. aureus strains for 1, 6, or 24 h.

Table 3. Changes of mRNA abundance (Mean ± SEM ΔΔCt¹) of immune factors in bMEC stimulated with six heat-inactivated S. aureus strains for 1, 6, or 24 h.