Table 1. Analysis of the reads for both the P7 and P3 libraries obtained following deep-sequencing of the circular conformers RT-PCR amplified from the total RNA of the infected tree.
Library | # of unfiltered reads | After filtering | After error correctionby KEC | After thresold removal | |||
Total # ofreads | # of unique reads | Total # ofreads | # of uniquereads | Total # ofreads | # of uniquereads | ||
P7 | 329,094 | 162,584 | 30,890 | 158,610 | 17,622 | 136,906 | 2,087 |
P3 | 458,584 | 176,564 | 25,914 | 172,829 | 14,358 | 155,053 | 1,852 |
Total | 787,678 | 339,148 | 56,804 | 331,439 | 31,980 | 291,959 | 3,939 |