Figure 4. Silencing of MCT4 decreases cell proliferation.

A. Proliferation of HN4 and HN6 cells was measured 96(siMCT4#1 and siMCT4#2) or a negative control (siNCtrl) by the CCK8 and SRB methods. Data represent mean and standard deviation of duplicates from three independent experiments. *: p<0.05. B. Lysates from identically treated cells were used to examine the protein expression levels of key proliferation-associated signaling molecules, as indicated, by western blot. MCT4 knock-down was also confirmed. β-actin was used as a loading control.