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. 2014 Jan 30;9(1):e88087. doi: 10.1371/journal.pone.0088087

Figure 5. Pharmacological AMPK activators control the association of B23, fibrillarin, nucleolin and RPA194 with the nucleus.

Figure 5

Kidney cells were incubated with vehicle (DMSO, D; Water, W), phenformin (Phen Ph), resveratrol (Res) or AICAR (AIC). Quantitative Western blotting was performed for nuclear and cytoplasmic fractions. (A) Blots were probed with antibodies against nucleolar proteins B23, fibrillarin, nucleolin or RPA194 and ECL signals were measured. The nuclear/cytoplasmic ratio was calculated; this ratio was defined as 1 for control samples. Bar graphs depict results as average+SEM for at least three independent experiments for each data point. *p<0.05; **p<0.01. (B) Control experiments evaluated the distribution of the cytoplasmic marker protein lactate dehydrogenase (LDH) and the nuclear protein lamin A.