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. 2014 Jan 30;9(1):e88087. doi: 10.1371/journal.pone.0088087

Figure 8. Effect of AMPK activators on lamin A and PARP1 cleavage.

Figure 8

After 1-h treatment with vehicle (D, DMSO; W, water), phenformin, resveratrol or AICAR, kidney cells were incubated with fresh medium for 24 h. In parallel, apoptosis was induced with staurosporine (STS); the vehicle DMSO served as negative control. At the end of the 24-h incubation period, attached and floating cells were combined for each sample, and crude extracts were probed with antibodies against lamin A, PARP1 or actin. Cleavage of lamin A and PARP1, which is associated with apoptosis, occured in staurosporine-treated cells, but not in response to phenformin, resveratrol or AICAR.