Table 1. The effects of phenformin, resveratrol, AICAR or cycloheximide on nucleolar proteins are summarized.

Protein	Compound	Nuc	Cyt	Nuc/Cyt	Nucleolus	No/Nuc	No/Cyt	Nuclear retention
B23	phenformin	↑	↑↑	↓	↔	↔	↓	↓
	resveratrol	↑	↑	↔	↑	↔	↔	↓
	AICAR	↔	↔	↔	↔	↔	↔	↑
	CHX	↔	↔	↑	↔	↔	↔	ND
Fibrillarin	phenformin	↓	↔	↓	↓	↔	↓	↓
	resveratrol	↔	↔	↔	↔	↔	↔	↓
	AICAR	↔	↔	↔	↔	↔	↔	↔
	CHX	↔	↔	↔	↔	↔	↔	ND
Nucleolin	phenformin	↑	↑↑	↓	↑	↔	↓	↓
	resveratrol	↑↑	↑↑	↔	↑↑	↔	↔	↓↓
	AICAR	↑	↑↑	↔	↔	↓	↓	↔
	CHX	↔	↔	↔	↔	↔	↔	ND
RPA194	phenformin	↔	↔	↔	↔	↔	↔	↓
	resveratrol	↔	↓	↑	↔	↔	↓	↓
	AICAR	↔	↔	↔	↓	↔	↓	↔
	CHX	↔	↔	↔	↔	↔	↔	ND
Nucleolar RNA synthesis	phenformin	↓
	resveratrol	↓↓
	AICAR	↓
p-H3	phenformin	↓
	resveratrol	↓
	AICAR	↓
DNA synthesis	phenformin	↓
	resveratrol	↓↓
	AICAR	↔

Results are depicted for individual compartments (nucleus, cytoplasm, nucleolus) or the distribution nucleus/cytoplasm (Nuc/Cyt), nucleolus/nucleus (No/Nuc) and nucleolus/cytoplasm (No/Cyt). The drug-induced reduction in de novo RNA synthesis is shown for comparison [14]. Results for control samples were defined as 1, and arrows denote the changes relative to controls: 0–0.24, ↓↓; 0.25–0.74, ↓; 0.75–1.24, ↔; 1.25–1.74, ↑; 1.75–2.24, ↑↑.