Table 4.
ADDITIVEa | SYNERGISTICb | |||||||
---|---|---|---|---|---|---|---|---|
Pathogen | N+P | N+E | E+P | N+P+E | N+P | N+E | E+P | N+P+E |
Listeria monocytogenes MTCC 657 | 50 + 600 | 50 + 700 | 600 + 700 | 50 + 600 + 700 | 25 + 300 | 25 + 350 | 300 + 350 | 25 + 300 + 350 |
E. faecium DSMZ 20477 | 53.5 + 70 | 53.5 + 937.5 | 937.5 + 70 | 53.5 + 70 + 937.5 | 26.25 + 35 | 26.25 + 468.75 | 468.75 + 35 | 26.25 + 35 + 468.75 |
E. faecium VRE | 14 + 2187.5 | 14 + 3750 | 2187.5 + 3750 | 14 + 2187.5 + 3750 | 7 + 1093 | 7 + 1875 | 1093 + 1875 | 7 + 1093 + 1875 |
N Nisin, P Pediocin 34, E Enterocin FH99
Units for concentrations: Nisin = IU/ml, Pediocin 34 = AU/ml, Enterocin FH99 = AU/ml
aCombination of bacteriocins corresponded to MICs of the target organisms
bCombination of bacteriocins correspond to Half the concentration of MICs of target organisms