Skip to main content
. 2011 Sep 2;51(2):233–244. doi: 10.1007/s13197-011-0500-3

Table 5.

Viable cell count (log cfu/ml) of L. monocytogenes and Enterococcus strains after treatment with nisin (N), pediocin 34 (P) and enterocin FH99 (E) alone and in different combinations

Culture Antibacterial effect of Bacteriocins when used alone ADDITIVE SYNERGISTIC
Incubation Time Control N P E N+P N+E E+P N+P+E N+P N+E E+P N+P+E
E. faecalis ATCC 29212 1 h 8.4 ± 0.01a,m 4.6 ± 0.06a,n ND ND ND ND ND ND ND ND ND ND
2 h 8.4 ± 0.03b,m 4.0 ± 0.01b,n ND ND ND ND ND ND ND ND ND ND
4 h 8.2 ± 0.07c,m 5.9 ± 0.03c,n ND ND ND ND ND ND ND ND ND ND
6 h 8.3 ± 0.01d,m 5.8 ± 0.09d,n ND ND ND ND ND ND ND ND ND ND
24 h 8.2 ± 0.07e,m 2.3 ± 0.03e,n ND ND ND ND ND ND ND ND ND ND
L. monocytogenes MTCC 657 1 h 7.6 ± 0.09a,m 6.9 ± 0.09a,n 5.7 ± 0.06a,o 6.8 ± 0.07a,p 5.3 ± 0.04a,q 4.8 ± 0.11a,r 3.0 ± 0.05a,s 6.0 ± 0.10a,t 3.8 ± 0.04a,u 3.4 ± 0.05a,v 2.3 ± 0.03a,w 3.4 ± 0.05a,x
2 h 7.5 ± 0.07b,m 6.9 ± 0.07b,n 5.9 ± 0.04b,o 6.5 ± 0.07b,p 5.2 ± 0.03b,q 5.2 ± 0.09b,r 3.6 ± 0.22b,s 5.0 ± 0.12b,t 3.9 ± 0.08b,u 4.3 ± 0.09b,v 2.3 ± 0.09b,w 3.5 ± 0.07b,x
4 h 7.6 ± 0.07c,m 7.0 ± 0.07c,n 5.3 ± 0.03c,o 7.3 ± 0.09c,p 5.7 ± 0.11c,q 5.3 ± 0.12c,r 3.9 ± 0.05c,s 7.0 ± 0.14c,t 3.6 ± 0.09c,u 4.0 ± 0.09c,v 2.4 ± 0.09c,w 4.1 ± 0.05c,x
6 h 8.6 ± 0.09d,m 6.8 ± 0.09d,n 6.1 ± 0.03d,o 7.5 ± 0.07d,p 6.0 ± 0.10d,q 4.0 ± 0.13d,r 3.8 ± 0.09d,s 7.8 ± 0.16d,t 4.3 ± 0.03d,u 2.9 ± 0.08d,v 2.1 ± 0.05d,w 4.5 ± 0.01d,x
24 h 7.1 ± 0.15e,m 6.8 ± 0.15e,m 5.6 ± 0.03e,o 6.4 ± 0.05e,p 5.1 ± 0.15e,q 4.2 ± 0.21e,r 2.4 ± 0.18e,s 4.3 ± 0.18e,t 4.5 ± 0.04e,u 4.5 ± 0.09e,v 2.0 ± 0.02e,w 3.9 ± 0.09e,x
E. faecium DSMZ 20477 1 h 6.9 ± 0.04a,m 5.8 ± 0.09a,n 6.7 ± 0.02a,m 6.1 ± 0.04a,p 6.8 ± 0.05a,m 6.2 ± 0.09a,r 6.5 ± 0.02a,s 5.5 ± 0.015a,t 3.0 ± 0.09a, u 4.6 ± 0.09a,v 4.6 ± 0.05a,w 2.3 ± 0.03a,x
2 h 8.2 ± 0.01b,m 6.5 ± 0.03b,n 6.9 ± 0.09b,o 7.3 ± 0.03b,p 6.8 ± 0.09b,q 6.1 ± 0.09b,r 5.3 ± 0.03b,s 4.9 ± 0.08b,t 3.3 ± 0.03b,u 3.4 ± 0.05b,v 4.4 ± 0.05b,w 2.3 ± 0.01b,x
4 h 7.6 ± 0.08c,m 7.4 ± 0.07c,m 7.6 ± 0.09c,m 7.3 ± 0.03c,m 7.0 ± 0.02c,q 6.9 ± 0.09c,r 6.0 ± 0.09c,s 5.3 ± 0.03c,t 3.0 ± 0.05c,u 3.3 ± 0.09c,v 5.4 ± 0.09c,w 2.2 ± 0.05c,x
6 h 8.5 ± 0.04d,m 5.7 ± 0.08d,n 7.4 ± 0.06d,o 8.3 ± 0.06d,p 7.1 ± 0.04d,q 6.1 ± 0.04d,r 7.3 ± 0.03d,s 6.2 ± 0.12d,t 5.3 ± 0.03d,u 4.0 ± 0.06d,v 6.3 ± 0.04d,w 3.0 ± 0.02d,x
24 h 8.9 ± 0.09e,m 8.0 ± 0.03e,n 8.4 ± 0.03e,o 8.5 ± 0.02e,p 8.3 ± 0.03e,q 8.3 ± 0.09e,r 8.2 ± 0.02e,s 7.3 ± 0.03e,t 5.3 ± 0.03e,u 5.0 ± 0.05e,v 7.6 ± 0.05e,w 2.3 ± 0.06e,x
E. faecium VRE 1 h 5.1 ± 0.21a,m 3.2 ± 0.04a,n 3.4 ± 0.09a,o 4.3 ± 0.06a,p 2.4 ± 0.11a,q 3.4 ± 0.22a,r 5.3 ± 0.12a,s 2.8 ± 0.11a,t 2.3 ± 0.03a,u 2.6 ± 0.09a,v 3.6 ± 0.09a,w 1.6 ± 0.06a,x
2 h 6.8 ± 0.03b,m 2.4 ± 0.02b,n 5.6 ± 0.03b,o 5.3 ± 0.04b,p 2.7 ± 0.22a,q 2.1 ± 0.14b,r 4.6 ± 0.08b,s 1.6 ± 0.10b,t 2.8 ± 0.09b,u 2.3 ± 0.01b,v 2.4 ± 0.09b,w 1.4 ± 0.06b,x
4 h 6.2 ± 0.12c,m 2.9 ± 0.05c,n 4.1 ± 0.05c,o 4.9 ± 0.05c,p 4.0 ± 0.23a,q 2.3 ± 0.04c,r 4.4 ± 0.07c,s 2.6 ± 0.19c,t 3.1 ± 0.01c,u 2.0 ± 0.03c,v 2.3 ± 0.09c,w 2.0 ± 0.09c,x
6 h 9.2 ± 0.02d,m 5.0 ± 0.03d,n 4.4 ± 0.09d,o 7.0 ± 0.12d,p 4.5 ± 0.31a,q 4.1 ± 0.13d,r 4.3 ± 0.12d,s 3.1 ± 0.02d,t 3.5 ± 0.02d,u 2.2 ± 0.03d,v 2.7 ± 0.02d,w 2.1 ± 0.02d,x
24 h 8.3 ± 0.07e,m 5.4 ± 0.03e,n 3.4 ± 0.07e,o 3.6 ± 0.08e,p 5.0 ± 0.12a,q 4.1 ± 0.02d,r 7.3 ± 0.03e,s 3.0 ± 0.21e,t 2.4 ± 0.12e,u 2.1 ± 0.02e,v 2.4 ± 0.05e,w 1.6 ± 0.09e,x

Values are mean±SE of three independent determinations (n = 3); ND Not detected. Values with different alphaphets, (a,b,c.d,e for incubation time and m,n,o,p,q,r,s,t,u,v,w,x for different bacteriocin treatments) are statistically significant (P < 0.05)