Fig. 5.

Embryonic mouse neural crest cells express Eda-A1. a Neural tube explant from E9.5 C57Bl/6J mouse embryos. Eighteen hours post-explantation, neural tubes were surrounded by a halo of thousands of emigrating and proliferating neural crest cells. b Cultures were fixed at 18 h post-explantation and processed for immunocytochemistry with antibodies against Eda-A1. All neural crest cells showed Eda-A1 immunoreactivity; subsets of cells were intensely immunoreactive. Asterisk indicates neural crest cells; nt neural tube. Number of explants stained and observed: 5. Bars, 50 μm