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. 2014 Feb;10(2):651–660. doi: 10.1016/j.actbio.2013.11.008

Fig. 2.

Fig. 2

Nuclear distortion analysis (OPG cells). Greyscale images captured in the DAPI channel (panel A) were exported and nuclei traced along their long and short axes (panels C and D). Background images where removed (panels E and F) and axis lengths calculated in ImageJ. The corresponding ratio gives a measure of nuclear elongation along the major axis. Panel B shows a complete RGB image: red is actin, green vinculin and blue DAPI (nuclear). Bar is 50 μm.