Supplementary Figure 1.

Validation of focal adhesion analysis (OPG cells). Greyscale images of immunolabelled adhesions labeled for paxillin were exported to Adobe Photoshop, and adhesions characterized as described in Fig. 1. Top panel shows RGB composite images of cells cultured on either planar or 540 nm-grooved PCL substrates. Red is actin, green paxillin and blue is nuclear; bar is 10 μm. Bottom panel shows focal adhesion subtypes and percentage subtype normalized to cell numbers (40 distinct cells). Relative proportions were similar to vinculin-based analysis, validating the use of vinculin as an adhesion marker. Statistical significance (t-test) relative to planar is noted (^*p < 0.05, ^**p < 0.01 and ^***p < 0.001).