Fig. 2.

Importin-β mRNA expression levels of mESCs differentiate into germ layer progenitors in vitro. (A) Phase-contrast images of EB3 cells exhibiting signs of differentiation following retinoic acid (RA) or CHIR99021 treatment. (B) RT-PCR expression analysis of early lineage markers Brachyury (for mesoderm) and Sox1 (for neural ectoderm). (C,D) Expression of Importin-βs in RA- or CHIR99021-treated mESCs was assessed by QPCR and is presented as a fold change relative to their expression levels in non-treated mESCs used as controls. Importin-βs mRNA levels were normalized to GAPDH levels. Significance was assessed and compared with the control using unpaired Student's t test (*p < 0.05; **p < 0.01). Error bars represent SEM from 3 experiments.