Fig. 5.

Expression of ME-specific markers from CHIR99021-treated Importin-β knockdown cells. (A) qPCR analysis of Brachyury in siRNA-treated EB3 cells incubated for 96 h in an enriched DMEM with LIF for the first 48 h and followed by incubation in CHIR99021- supplemented, LIF-withdrawn enriched DMEM for the next 48 h. All samples were normalized against GAPDH levels, and expression of Brachyury in siRNA-treated EB3 cells is shown as a fold change relative to its expression in non-targeting siRNA treated EB3 cells used as control. Significance was assessed and compared with the control using unpaired Student's t test (*p < 0.05; **p < 0.01). Error bars represent SEM from 4 independent experiments. (B) qPCR analysis of FoxA2 as performed in (A).