Fig. 3.

Autophosphorylation of WalK and its variants, with its intensity analysis (ImageJ software). Autophosphorylation was observed within 5 min of γ-³²P-ATP addition and increased with time for each variant as shown. (A) Autophosphorylation of WalKc. (B) Autophosphorylation of WalKc(PH) with WalKc as a positive control. (C) Autophosphorylation of WalKc(H) with WalKc and WalKc(PH) as the positive controls. The reaction mixtures were analyzed by a 10% SDS–PAGE, followed by autoradiography. For each autoradiogram, intensity units (%) for phosphorylation are plotted against time in minutes.