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Phosphotransfer from WalK variants to WalR, with its intensity analysis (ImageJ software). Phosphotransfer was observed within 5 min of γ-32P-ATP addition and increased with time as shown. (A) WalKc to WalR phosphotransfer. (B) WalKc(PH) to WalR phosphotransfer. (C) WalKc(H) to WalR phosphotransfer. The reaction mixtures were analyzed by a 10% SDS–PAGE, followed by autoradiography. For each autoradiogram, intensity units (%) for phosphotransfer are plotted against time in minutes.
