Fig. 8.

DNA binding ability of WalR. EMSA was used to check for WalR binding to DNA by incubating increasing amounts of purified WalR (2, 3 and 4 μg) with eag, ftsE and kinB3 probes containing the WalR binding site sequence. IS probe, lacking the WalR binding site sequence, was the negative control. Binding reactions were performed at room temperature and the reaction products were run on 8% polyacrylamide native gels in 0.5X TBE at 4 °C. Bands were detected after transfer to a positively charged nylon membrane using a chemiluminescent nucleic acid detection module. WalR could bind to eag, ftsE and kinB3 probes, but not IS probe as shown.