Fig. 4.

gdh3-dependent ROS generation by ebselen in S. cerevisiae. Wild-type and the gdh1, gdh2 and gdh3-deletion mutant were treated with 20 μM ebselen for 2 h. Then, they were stained with 10 μM H₂DCFDA for 1 min, and the level of ROS was observed by fluorescence microscopy. (A) Background ROS levels in WT, gdh1, gdh2 and gdh3-deletion mutants. Upper panels show phase contrast microscopy; the lower panels show florescence microscopy of the same cells. (B) ROS level in mutant and wild-type cells.