Figure 7.

Temperature driven changes in sEPSC frequency across the physiological range are not different from TRPV1 KO afferents. (A) Current traces of temperature driven increase in sEPSC frequency from TRPV1+ and TRPV1 KO mice. (B) Scatter-plot showing binned average frequency (10 s bins) across changing temperatures from the TRPV1+ and TRPV1 KO neurons shown in (A). (C) Frequency of sEPSCs at each temperature from individual recorded neurons from TRPV1+ and TRPV1 KO animals. Lines connect frequencies at each temperature for an individual neuron. (D) Average sEPSC frequencies across neurons at each of the steady state temperatures. (E) Changes in frequency normalized to frequency recorded at 33°C. The frequency of spontaneous release is still temperature driven in the absences of TRPV1.