Fig. 2.

Intracellular distribution of WT1-pTj in A2058 human melanoma cells. (A) Cells were treated with FITC-labeled WT1-pTj for 1 h at 37 °C and visualized by confocal microscopy. Sequence (Z-stacks) showing the distribution of FITC-labeled peptide (green) and the nuclei stained with DAPI (blue). Bar = 10 μm. (B) Co-localization of WT1-pTj and nuclear DNA in human melanoma cells. A2058 cells were treated with biotinylated WT1-pTj for 24 h, fixed with paraformaldehyde, and permeabilized with Triton X-100. (a) Differential interference contrast (DIC) image; (b) Cells stained with DAPI; (c) Cells treated with streptavidin-FITC; (d) Merge, showing nuclear colocalization of WT1-pTj. Inserts: high magnification of cells indicated by arrows. Scale bar = 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)