Fig. 3.

Effects of WT1-pTj on proliferation and clonogenic ability of human melanoma cells. (A) A2058 cells (10³/well) were treated with WT1-pTj (left) or C PEP (right) at the indicated concentrations for 96 h, and cell proliferation was daily monitored by Trypan blue exclusion assay; (B) A2058 cells were suspended in 300 μl of 0.5 mM WT1-pTj, 0.5 mM C PEP, or unsupplemented culture medium (Control) and cultivated on soft-agar medium. The number of colonies was determined 11 days after the first treatment; ^*p < 0.02. (C) Representative photomicrographs of colony formation assay. A2058 cells were plated on (a) untreated culture medium (Control), (b) medium treated with 0.5 mM C PEP, or (c) treated with 0.5 mM WT1-pTj. After 5 days, culture media were aspirated and fresh medium with the respective peptides at 0.5 mM was added. After 10 days incubation, colonies were stained with crystal violet and counted on a stereomicroscope. Data represent means ± SD of three independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)