Fig. 5.

Effect of WT1-pTj on cell cycle progression and modulation of p53, and downstream mediators, in human melanoma cells. (A) A2058 cells were treated either with 0.5 mM WT1-pTj or 0.5 mM C PEP, for 24 h, and the cell cycle profile was examined by flow cytometry. Control, unsupplemented culture medium. Results are representative of three independent experiments. (B) Cellular extracts from A2058 cells exposed to 0.5 mM WT1-pTj or 0.5 mM C PEP were subjected to immunoblotting with antibodies specific for p53, phospho-p53, p27, p21, phospho-cdc2 and cyclin B1. Protein load was normalized to β-actin.