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. 2014 Jan 21;4:147–152. doi: 10.1016/j.fob.2014.01.006

Fig. 1.

Fig. 1

BH1-BH2 but not the PEST domain has a short half-life. (A) Schematic diagram of GFP-Mcl-1-ΔPEST, GFP-NsMcl-1, GFP-PESTMcl-1, GFP-BH3Mcl-1 and GFP-BH1-BH2Mcl-1. (B) Western blotting showing the protein amount of over-expressed GFP-Mcl-1-ΔPEST and GFP-PESTMcl-1 at different time points after UV irradiation in HeLa cells. *The protein amount of tubulin served as the loading control. (C) Western blotting showing the protein amount of over-expressed GFP-NsMcl-1, GFP-BH3Mcl-1 and GFP-BH1-BH2Mcl-1 at different time points after UV irradiation in HeLa cells. *The protein amount of tubulin served as the loading control.