The expression of GFP fused F3 single residue deletion mutants in HeLa cells. (A) Phase contrast and fluorescent images of GFP-F3 (VTLISFG), GFP-TLISFG, GFP-VLISFG, GFP-VTISFG, GFP-VTLSFG, GFP-VTLIFG, GFP-VTLISG, and GFP-VTLISF in HeLa cells. (B) The expression of F3 mutants determined by Western blotting analysis in HeLa cells. The endogenous cdc2 served as the loading control.