Fig. 3.

Protein levels of Akt, ERK1/2, and cleaved caspase 3 of isolated pancreatic islets in SHAM, SHL, OVX, OHL and OHLD groups. Panel A is the immunobloting of pAkt, Akt, ERK1/2, cleaved caspase 3 and α-tubulin. Panel B is the stoichiometric relationship between the serine phosphorylation status of Akt (p-Akt) and the protein expression of Akt (pAkt/Akt). Panel C is the protein level of ERK1/2. Panel D is the protein level of cleaved caspase-3. The values are expressed as the mean ± S.E.M. and distinct letters indicate significance (P < 0.05).