Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 31;5:13. doi: 10.3389/fpls.2014.00013

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Zientara-Rytter and Sirko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cytoplasmic and vacuolar localization of transiently expressed Joka2 and AtNBR1 in leaf epidermal cells of N. benthamiana. (A) BiFC assay of interaction between Joka2 and NtATG8f using randomly chosen combination (YN-NtATG8f+Joka2-YC) of the vectors. (B) Co-localization of co-expressed GFP-AtNBR1 and RFP-AtNBR1 (AtNBR1 fused to two variants of fluorescent protein). (C) Localization of Joka2-RFP in the vacuole. (D) Subcellular localization of co-expressed RFP-Joka2 and γ-TIP-CFP—a tonoplast marker based on an aquaporin of the vacuolar membrane fused to CFP. The enlarged part of the picture visualizes tonoplast (the blue fluorescence signal) of the central vacuole which surrounds the nucleus (N) and red fluorescence of RFP-Joka2 fusion protein observed mainly inside the vacuole close to the tonoplast as a smear (arrowheads) or in spots (arrows). Scale bar, 10 μm.