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. 2014 Jan 31;5:13. doi: 10.3389/fpls.2014.00013

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Copyright © 2014 Zientara-Rytter and Sirko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between PB1 and UBA domains. (A) Yeast two-hybrid (Y2H) analysis demonstrating weak interaction between PB1 domain and the fragment containing UBA domains of Joka2. Truncated proteins lacking either PB1 or UBA domains were fused to AD or BD domain of GAL4 protein and co-expressed in yeast cells (AH109 strain). Positive and negative controls for protein interaction analysis were provided by Invitrogen. (B) BiFC assay for PB1-UBA interaction in planta. Indicated combinations of plasmids (PB1-YN+UBA-YC, YN-PB1+UBA-YC, YC-PB1+UBA-YN, PB1-YC+UBA-YN) were used for analysis. Scale bar, 10 μm.