Figure 3.

The C-terminal region of G72 interacts with DAO. (A) Upper panel. Only yeast co-transformed with plasmids containing the genes for AD-DAO and BD-G72 were viable on SD/-2 and SD/-4 plates. Yeast co-transformed with AD-DAO and a truncated form of the BD-G72 gene was not viable on SD/-4 plates. Lower panel. Western blotting to confirm that AD-DAO and BD-G72 and truncated forms of BD-G72 were expressed in the yeast system; and (B) The relative strengths of the interactions between DAO and G72 or a truncated form of G72 were determined by the level of β-galactosidase activity and normalized to the activity in yeast co-transformed with the AD and BD tags. *** p < 0.001.