Table 4.

mRNA expression levels in in vitro study.

Item	Group	Monolayer Culture		Spheroid Culture
		24 h (1) (Ratio to the Control)	48 h (1) (Ratio to the Control)	24 h (1) (Ratio to the Control)	48 h (1) (Ratio to the Control)
hCYP1A1/hGAPDH	Control	0.022 ± 0.033 (1.0)	0.033 ± 0.054 (1.0)	0.018 ± 0.021 (1.0)	0.013 ± 0.007 (1.0)
	3-MC 10 ng/mL	0.053 ± 0.060 (2.4)	0.038 ± 0.056 (1.2)	0.047 ± 0.035 (2.6)	0.023 ± 0.018 (1.8)
	3-MC 50 ng/mL	0.112 ± 0.101 (5.1)	0.053 ±0.053 (1.6)	0.120 ± 0.073 (6.6)	0.053 ± 0.028 (4.2)
	3-MC 250 ng/mL	0.314 ± 0.170 * (14.3)	0.158 ± 0.086 (4.9)	0.315 ± 0.175 ** (17.3)	0.186 ± 0.087 (14.9)
hCYP1A2/hGAPDH	Control	0.007 ± 0.006 (1.0)	0.003 ± 0.004 (1.0)	0.139 ± 0.091 (1.0)	0.168 ± 0.062 (1.0)
	3-MC 10 ng/mL	0.015 ± 0.011 (2.3)	0.004 ± 0.004 (1.4)	0.215 ± 0.106 (1.6)	0.228 ± 0.056 (1.4)
	3-MC 50 ng/mL	0.017 ± 0.008 (2.5)	0.006 ± 0.004 (1.8)	0.392 ± 0.069 ** (2.8)	0.423 ± 0.117 * (2.5)
	3-MC 250 ng/mL	0.033 ± 0.012 * (4.9)	0.015 ± 0.004 ** (4.9)	0.713 ± 0.023 ** (5.1)	0.967 ± 0.111 ** (5.8)
hCYP3A4/hGAPDH	Control	0.019 ± 0.004 (1.0)	0.004 ± 0.000 (1.0)	0.278 ± 0.240 (1.0)	0.116 ± 0.070 (1.0)
	Rifampicin 5 μg/mL	0.075 ± 0.044 * (4.0)	0.040 ± 0.020 * (10.9)	4.138 ± 0.405 ** (14.9)	3.421 ± 1.601 * (29.4)
	Rifampicin 25 μg/mL	0.187 ± 0.061 * (9.8)	0.055 ± 0.025 * (15.1)	4.952 ± 1.294 ** (17.8)	2.770 ± 0.907 * (23.8)

⁽¹⁾mean ± S.D.;

^*p < 0.05;

^**p < 0.01.