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. 2013 Dec 27;15(1):261–276. doi: 10.3390/ijms15010261

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — E_app histograms of a cell calculated from the FRET efficiency map as measured using the line-scan system (a) and the point scan system (b) after repeated scans of the sample along with the normalized emission of the donors corrected for FRET. Pixels for which either k^DA or k^AD values were within less than 2 standard deviations of the noise level were discarded from the analysis. Blue solid line: scan 1; green dashed line: scan 4; red dashed-dotted line, scan 7. The maximum values for the F^D were 38,400 and 14,775 for (a) and (b) respectively, for these cells.