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. 2014 Jan 6;15(1):545–559. doi: 10.3390/ijms15010545

Figure 2.

Figure 2.

IL-6 increases cell migration by upregulating intercellular adhesion molecule-1 (ICAM-1). (A) Cells were pretreated for 30 min with ICAM-1 mAb (10 μg/mL) or transfected with ICAM-1 small interfering RNA (siRNA) for 24 h, followed by stimulation with IL-6 (30 ng/mL). In vitro migration activity was measured with the Transwell assay (n = 5); (B–D) SCC4 cells were incubated with IL-6 for 24 h, and ICAM-1 expression was examined by flow cytometry, quantitative real-time polymerase chain reaction (qPCR), and western blotting (n = 6); and (E,F) Protein levels and migratory activity of IL-6 and ICAM-1 in SCC4/control short hairpin RNA (shRNA) and SCC4/IL-6 shRNA cells were examined by western blotting and the Transwell assay (n = 5). Results are expressed as the mean ± SEM; *, p < 0.05 compared with the control; #, p < 0.05 compared with the IL-6-treated group.