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. 2014 Jan 6;15(1):545–559. doi: 10.3390/ijms15010545

Figure 5.

Figure 5.

JNK is involved in IL-6-induced migration and ICAM-1 expression. (A–C) Cells were pretreated for 30 min with SP600125 (3 μM) or transfected with the JNK mutant for 24 h and stimulated with IL-6 (30 ng/mL) for 24 h. In vitro migration and ICAM-1 expression were measured using the Transwell, wound healing, and qPCR assays (n = 5); (D) SCC4 cells were incubated with IL-6 (30 ng/mL) for the indicated time intervals, and JNK phosphorylation was examined by western blotting (n = 5); and (E) SCC4 cells were pretreated for 30 min with IL-6R mAb or the Syk inhibitor and stimulated with IL-6 (30 ng/mL) for 30 min; Syk phosphorylation was determined by western blotting (n = 4). Results are expressed as the mean ± SEM; *, p < 0.05 compared with the control; #, p < 0.05 compared with the IL-6-treated group.