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. 2014 Jan 6;15(1):545–559. doi: 10.3390/ijms15010545

Figure 6.

Figure 6.

Activator protein 1 (AP-1) is involved in IL-6-mediated migration in human OSCC cells. (A–C) Cells were pretreated for 30 min with curcumin (10 μM) and tanshinone (10 μM) or transfected for 24 h with c-Jun siRNA followed by stimulation with IL-6 for 24 h. In vitro migration and ICAM-1 expression were measured using the Transwell, wound healing, and qPCR assays (n = 5); (D) SCC4 cells were incubated with IL-6 (30 ng/mL) for the indicated time intervals, and c-Jun phosphorylation was examined by western blotting (n = 4); and (E–G) SCC4 cells were pretreated for 30 min with IL-6R mAb, the Syk inhibitor, or SP600125 for 30 min followed by stimulation with IL-6 for 60 min. c-Jun phosphorylation, c-Jun binding to the AP-1 element, and c-Jun translocation into the nucleus were determined by western blotting, ChIP, and immunofluorocytochemistry (n = 5). Results are expressed as the mean ± SEM; *, p < 0.05 compared with the control; #, p < 0.05 compared with the IL-6-treated group.