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. 2014 Jan 8;15(1):758–773. doi: 10.3390/ijms15010758

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Enriched exosomal microRNA-146a in cervicovaginal lavage specimens of cervical cancer patients. (A) The relative amounts of miR-146a were examined by RT-qPCR in cervicovaginal lavage specimens of the HPV-negative normal subjects (n = 32), the HPV-positive subjects (n = 25), or the cervical cancer patients (n = 45). The lowest expression in the normal (HPV-) group was designated as “1”. All other samples were expressed as a relative value according to the ratio of their Cp value to the Cp value of the designated sample; (B–D) The correlation between the miR-146a expression and the level of exosomes was evaluated by RT-qPCR and ELISA; (B) HPV–negative normal group; (C) HPV-positive group; (D) Cervical cancer patient group. All experiments were performed in triplicate. Statistical significance was considered with a p < 0.05 (*) and a p < 0.01 (**).