Figure 3.

Gram’s iodine detection of cellulase activity in tissue prints covered with a layer of gelatinous CMC. (A–C) Cellulase activity in longitudinal sections prepared from stems of (from left) Solanum lycopersicum, Cuscuta reflexa and Pelargonium zonale; and (D–H) Localization of cellulase activity in cross-sections of parasitic plant infection sites. For ease of reference host and parasite (including the haustorium penetrating into the host) have been encircled with dark green and light green lines, respectively; (A,D) plant material as printed; (B,E,G) tissue prints on dry nylon membrane; and (C,F,H) zymograms after coating with 4% CMC and Gram’s iodine staining.