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. 2014 Jan 17;15(1):1201–1215. doi: 10.3390/ijms15011201

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 6. — Effects of hispolon on apoptosis in B16-F10 and Detroit 551 cells. The cells were stained with fluorescein isothiocyanate (FITC)-labeled annexin-V/propidium iodide (PI) double stain, and the percentages of apoptotic and necrotic cells were calculated. (A) untreated B16-F10 cells; (B) 10 μM camptothecin treated B16-F10 cells; (C) untreated Detroit 551 cells; (D) B16-F10 cells treated with 10 μM of hispolon; (E) B16-F10 cells treated with 30 μM of hispolon; (F) B16-F10 cells treated with 50 μM of hispolon, (G) Detroit 551 cells treated with 10 μM of hispolon; (H) Detroit 551 cells treated with 30 μM of hispolon; and (I) Detroit 551 cells treated with 50 μM of hispolon.