Figure 3.

Profile of DNase production in the presence of DNA or deoxyribose. All flasks were incubated for five days (120 h) in the absence of exogenous DNA with agitation (100 RPM). After the fifth day, 1 g of filter sterilized DNA (10 mL) or 1 g deoxyribose was added to a set of flasks and incubated with agitation for 50 h at 55 °C. Control flasks were similarly incubated without the addition of DNA. Activity was measured using the acid soluble assay with a 60 min. incubation time at 55 °C. All assays were performed in triplicate using triplicate flasks.